Table 8: DNA/methyl green colorimetric assay of the DNA-binding compounds.


DNA-active compound DNA/methyl green (IC50, μg/mL)

DOX 31.27 ± 1.8
L 29.18 ± 1.6
Pt(IV) 82.27 ± 2.7
Cu(II) 38.02 ± 1.9
Cd(II) 45.35 ± 2.1
Ni(II) 34.71 ± 1.8

IC50 values represent the concentration (mean ± SD, n = 3–5 separate determinations) required for a 50% decrease in the initial absorbance of the DNA/methyl green solution.