Table 2:
PCR parameters for linearizing the pGL3-U6-sgRNA-PGK-puroR vector.
Cycle
Denaturation
Annealing
Extension
1
98 °C, 30 sec
2–36
98 °C, 10 sec
69 °C, 20 sec
72 °C, 2.5 min
37
72 °C, 2 min