Table 4:
Colony PCR parameters.
Cycle
Denaturation
Annealing
Extension
1
95 °C, 6 min
2–7
95 °C, 30 sec
65 °C, 30 sec
(1 °C less each cycle)
68 °C, 44 sec
8–31
58 °C, 30 sec
68 °C, 44 sec
32
68 °C, 8 min